![]() ![]() Depending on their genomic location, lncRNAs can be classified into genic lncRNAs (overlapping with a protein-coding gene) or intergenic lncRNAs (lincRNAs no overlap with a protein-coding gene) ( Ransohoff et al., 2018). ![]() Several of those have been shown to influence cellular physiology in developmental, adult and disease contexts ( Sarropoulos et al., 2019 James, 2015 Prensner et al., 2011 Castellanos-Rubio et al., 2016 Perry and Ulitsky, 2016 Lorenzi et al., 2021). ![]() To date, nearly 28,000 long non-coding RNAs (lncRNAs) have been reported in the human genome, but less than 1% (~150) has been functionally characterized ( Ransohoff et al., 2018 Hon et al., 2017 Quek et al., 2015 Jiang et al., 2016). Taken together, our study identified and characterized T-REX17 as a transiently expressed and essential non-coding regulator in early human endoderm differentiation. Consequently, cells lacking the lncRNA cannot further differentiate into more mature endodermal cell types. Loss of T-REX17 affects crucial functions independent of SOX17 and leads to an aberrant endodermal transcriptome, signaling pathway deregulation and epithelial to mesenchymal transition defects. We termed it T-REX17 ( Transcript Regulating Endoderm and activated by so X17) and show that it is induced following SOX17 activation but its expression is more tightly restricted to early definitive endoderm. Here, we report the discovery of a previously unannotated lncRNA that is transcribed 230 kb upstream of the SOX17 gene and located within the same topologically associating domain. Despite much progress over the past decade, the genome-wide annotation of lncRNAs remains incomplete and many known non-coding loci are still poorly characterized. Long non-coding RNAs (lncRNAs) have emerged as fundamental regulators in various biological processes, including embryonic development and cellular differentiation. The Ludwig Center at Harvard, Boston, MA 02215, USA, and Center for Cancer Evolution, Dana-Farber Cancer Institute, United States.Chan School of Public Health, United States Department of Data Science, Dana-Farber Cancer Institute, and Department of Biostatistics, Harvard T.Broad Institute of MIT and Harvard, United States.Department of Stem Cell and Regenerative Biology, Harvard University, United States.Diabetes Center of Excellence, University of Massachusetts Medical School, United States.Max Planck Institute for Molecular Genetics, Mass Spectrometry Core Facility, Germany.Program in Molecular Medicine, University of Massachusetts Medical School, United States.Center for Precision Medicine Multi-Omics Research, School of Basic Medical Sciences, Peking University Health Science Center and Peking University Cancer Hospital and Institute, China.Department of Data Science, Dana-Farber Cancer Institute, Department of Biostatistics, Harvard T.Helmholtz Institute for Metabolic, Obesity and Vascular Research, Germany.Max Planck Institute for Molecular Genetics, Microscopy Core Facility, Germany.Department of Biochemistry, University of Colorado Boulder and BioFrontiers Institute, United States.Institute of Chemistry and Biochemistry, Freie Universität Berlin, Germany.Department of Genome Regulation, Max Planck Institute for Molecular Genetics, Germany. ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |